Effect of Enzymatic Functional Group Inhibitors on Proteolytic Enzymes of Crotalus Atrox Venom. Rep No. 622.

نویسنده

  • J H BROWN
چکیده

EFFECT OF El7MTI FUNC.T]DN'AL GROUP INBI3ITORS ON PRGIE)0LTELYT ZYBMKU OF TA-3 S ATMO' WIEi1N C C43T To determine the effects of several functionaL group,-specific inhib=Qrsof enzymes and: of metal ioneon. the activityof the proteolytic enzymes of Crotalusatrmx venom.. To attempt M discern the typeand: number of proteolytic enzymes presenti m crude venom and: to obtain & detoxified,, yet immu.nologically active venom. Only cas-keinoLytic activity seemsto beappreiahly inhibited. by sulfhydry' group -specifl reagents-.. All proteaesare inhihited by thioL-contaming compounds-.. Dis-oproyslu rphophateihbhit% only proteolytic actiavitywith N-benzoyb-I -azginine-p.-nifr-oanilide a,& subs-trate.. N[-hromos-ucinimide. inhibits only proteolytic activity on cungocoll and: N-benzyL-D-arginine-p-nitr-oanilice. Ethylenediaminetetraacetate inhibits pr-oteolytic activity on all substraresk tested except LN-benzoyL-DL-ar-ginme-p-nitroanilide.. a-Amino-n.-caproic acid en,hancesca-seinolysiat Low concentrations and. inhibits at higher concentrations-. Calcium ions anc other divalent alkaline earths have a general. actrivating effect on. proteoLytic activity of venom.. Reavy metal ions-,, in. general,. have an. inhibitory effect on. proteolytic &ctivty..

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عنوان ژورنال:
  • Report. Army Medical Research Laboratory

دوره   شماره 

صفحات  -

تاریخ انتشار 1965